Rabbit Anti-Human Wnt-3a Polyclonal Antibody

• Description:

  Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. During development, Wnt-3a is a morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination. When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation. Wnt-3a has also been implicated in chondrocyte differentiation. Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity. A second site at Ser 209 modified by palmitoleic acid also contributes. Human Wnt-3a shares 96% amino acid (aa) identity with mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt-3. Human Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues.

• Synonyms:

  WNT3A

• NCBI Gene ID:

  89780

• UniProt:

  P56704

• Accession Number:

  NP_149122

• Accession Number mRNA:

  NM_033131

• Gene Location:

  1q42

• Host:

  Rabbit

• Origin Species:

  Human

• Species Reactivity:

  Human

• Target Antigen:

  Recombinant Human WNT-3a

• Clone:

  Rabbit IgG

• Validated Applications:

  ELISA, WB

• Purification Method:

  Antigen-affinity purified

• Form:

  Lyophilized from PBS

• Buffer:

  PBS

• Reconstitution:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.

• CAS Number:

  9007-83-4