Wnt-3a

• Description:

  Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. During development, Wnt-3a is a morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination. When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation. Wnt-3a has also been implicated in chondrocyte differentiation. Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity. A second site at Ser 209 modified by palmitoleic acid also contributes. Human Wnt-3a shares 96% amino acid (aa) identity with mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt-3. Human Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues. Recombinant Human Wnt-3a consists of 342 amino acid residues, including a C-terminal His-Tag and has a molecular weight of 38.6 kDa.

• Synonyms:

  Wnt-3a

• NCBI Gene ID:

  89780

• UniProt:

  P56704

• Accession Number:

  NP_149122

• Accession Number mRNA:

  NM_033131

• Chromosomal Location:

  1q42

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  His-Tag

• Sequence:

  MSYPIWWSLAVGPQYSSLGSQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHTCKLEHHHHHH

• Assay Protocol:

  Human Wnt3a should be reconstituted in 50 mM acetic acid to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.

• Purity:

  > 85% by SDS-PAGE & Coomassie stain

• Length:

  343

• Form:

  Lyophilized

• Reconstitution:

  50 mM acetic acid

• Molecular Weight:

  38.6 kDa

• Storage Conditions:

  The lyophilized human Wnt3a, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted human Wnt3a should be stored in working aliquots at -20°C.

• Host or Source:

  E. coli

• N Terminal Sequence:

  SYPIW