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Wnt-3a

CAT: 0209-400-022Size: 25 µgDry Ice: NoHazardous: No
CAT#:0209-400-022Size:25 µg
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Description
Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. During development, Wnt-3a is a morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination. When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation. Wnt-3a has also been implicated in chondrocyte differentiation. Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity. A second site at Ser 209 modified by palmitoleic acid also contributes. Human Wnt-3a shares 96% amino acid (aa) identity with mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt-3. Human Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues. Recombinant Human Wnt-3a consists of 342 amino acid residues, including a C-terminal His-Tag and has a molecular weight of 38.6 kDa.
Synonyms
Wnt-3a
NCBI Gene ID
89780
UniProt
P56704
Accession Number
NP_149122
Accession Number mRNA
NM_033131
Chromosomal Location
1q42
Reactivity
Human
Cross Reactivity
Human
Label
His-Tag
Sequence
MSYPIWWSLAVGPQYSSLGSQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHTCKLEHHHHHH
Assay Protocol
Human Wnt3a should be reconstituted in 50 mM acetic acid to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.
Purity
> 85% by SDS-PAGE & Coomassie stain
Length
343
Form
Lyophilized
Reconstitution
50 mM acetic acid
Molecular Weight
38.6 kDa
Storage Conditions
The lyophilized human Wnt3a, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted human Wnt3a should be stored in working aliquots at -20°C.
Host or Source
E. coli
N Terminal Sequence
SYPIW

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