Wnt-5a

• Description:

  Wnt-5a is one of the most highly investigated non-canonical Wnts and has been implicated in almost all aspects of non-canonical Wnt signalling. In terms of cancer development, Wnt-5a has, until recently, lived in the shadow of its better-characterised relatives. This was largely because of its apparent inability to transform cells or signal through the canonical beta-catenin pathway that is so important in cancer, particularly colorectal cancer. Recent work in a wide range of human tumours has pointed to a critical role for Wnt-5a in malignant progression, but there is conflicting evidence whether Wnt-5a has a tumour-promoting or -suppressing role. Emerging evidence suggests that the functions of Wnt-5a can be drastically altered depending on the availability of key receptors. Hence, the presence or absence of these receptors may go some way to explain the conflicting role of Wnt-5a in different cancers.

• Synonyms:

  Wnt-5a; Wnt5a; Wnt 5a; Wnt

• NCBI Gene ID:

  7474

• UniProt:

  P41221

• Accession Number:

  NP_003383.2

• Accession Number mRNA:

  NM_003392.4

• Chromosomal Location:

  3p14-p21

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  His-Tag

• Sequence:

  MIIGAQPLCSQLAGLSQGQKKLCHLYQDHMQYIGEGAKTGIKECQYQFRHRRWNCSTVDNTSVFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELSTCGCSRAARPKDLPRDWLWGGCGDNIDYGYRFAKEFVDARERERIHAKGSYESARILMNLHNNEAGRRTVYNLADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNSRGKLVQVNSRFNSPTTQDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTERCHCKFHWCCYVKCKKCTEIVDQFVCKLEHHHHHH

• Applications:

  No direct biological data available at the moment but human Wnt5a binds to immobilized human sROR2 in a functional ELISA.

• Assay Protocol:

  Human Wnt5a should be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.

• Purity:

  > 85% by SDS-PAGE & Coomassie stain

• Length:

  328

• Form:

  Lyophilized

• Buffer:

  50 mM acetic acid

• Reconstitution:

  Water

• Molecular Weight:

  36.9 kDa

• Storage Conditions:

  The lyophilized human Wnt5a, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted human Wnt5a should be stored in working aliquots at -20°C.

• Host or Source:

  E. coli

• N Terminal Sequence:

  MIIGA