VEGFR-1/Flt-1, Agonistic

• Description:

  Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs) . They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4) . Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The antibody will bind near the ligand binding site of the receptor and has agonistic activity.

• Synonyms:

  Vascular endothelial growth factor receptor-1; FLT1; FLT; VEGF receptor 1

• NCBI Gene ID:

  2321

• UniProt:

  P17948

• Accession Number:

  NP_001153392

• Accession Number mRNA:

  NM_001159920

• Chromosomal Location:

  13q12

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  HVEGFR1 N-terminal fragment (N25-250)

• Clone:

  (#8F12)

• Applications:

  WB, Stimulation

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge the vial prior to opening. Reconstitute the antibody with 200 µl sterile PBS and the final concentration is 500 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS (sterile)

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG1