VEGFR-1/Flt-1

• Description:

  VEGFR-1 is a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig) -like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.

• Synonyms:

  Vascular endothelial growth factor receptor-1; FLT1; VEGF receptor 1

• NCBI Gene ID:

  2321

• UniProt:

  P17948

• Accession Number:

  NP_001153392

• Accession Number mRNA:

  NM_001159920

• Chromosomal Location:

  13q12

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human, mouse

• Target Antigen:

  Recombinant human soluble Flt-1 (D5)

• Clone:

  (#EWC)

• Applications:

  ELISA, WB, IF

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  ELISA: Use 1–10 µg/ml; Western blotting: Use at 2–5 µg/ml; The monoclonal antibody will detect native and denatured human VEGFR-1/Flt-1 in ELISA experiments and on the surface of different human cell types.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Notes:

  The antibody will detect native human VEGFR-1/Flt-1 in ELISA experiments and on the surface of different human cell types.

• Host or Source:

  Mouse

• Isotype:

  IgG1