VEGFR-1/Flt-1

• Description:

  Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs) . They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4) . Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.

• Synonyms:

  Vascular endothelial growth factor receptor-1; FLT1; VEGF receptor 1

• NCBI Gene ID:

  2321

• UniProt:

  P17948

• Accession Number:

  NP_001153392

• Accession Number mRNA:

  NM_001159920

• Chromosomal Location:

  13q12

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human soluble extracellular Flt-1 (D1-D5)

• Clone:

  (#EWF)

• Label:

  Biotin

• Applications:

  ELISA, WB, IP

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  ELISA: Use 1–10 µg/ml; Western blotting: Use 2–5 µg/ml; Immunoprecipitation: Use 2-10 µg/ml

• Form:

  Lyophilized

• Buffer:

  PBS

• Additives:

  BSA (50x), 0.02% sodium azide

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Notes:

  The unconjugated antibody can be used for ELISA experiments, Western blotting, immunofluorescence, and immunoprecipitation. It will recognize recombinant and naturally occurring form of human VEGFR-1/Flt-1.

• Host or Source:

  Mouse

• Isotype:

  IgG1