VEGF-C

• Description:

  VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The human VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant human VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant human VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant human VEGF-C contains 115 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.

• Synonyms:

  Vascular endothelial growth factor C; VEGFC; VRP; Flt4-L

• NCBI Gene ID:

  7424

• UniProt:

  P49767

• Accession Number:

  NP_005420.1

• Accession Number mRNA:

  NM_005429.2

• Chromosomal Location:

  11q13

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human, rat

• Target Antigen:

  Recombinant human VEGF-C (RT# 300-079)

• Clone:

  (#9E7)

• Applications:

  WB, IHC (F)

• Purification Method:

  Protein G affinity chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  Western Blot: Use at 1-5 µg/ml; IHC: Use at 2-10 µg/ml

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Notes:

  This antibody detects human and rat VEGF-C in Western blots. No cross-reactivity is shown with VEGF-A.

• Host or Source:

  Mouse

• Isotype:

  IgG2a