Products for Research Use Only
RegisterLogin

TNF-alpha

CAT: 0209-103-PA09Size: 200 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0209-103-PA09Size:200 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Tumor necrosis factor alpha (TNFα), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNFα is produced by a wide variety of immune and epithelial cell types. Mouse TNFα consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) . Within the ECD, mouse TNFα shares 94% aa sequence identity with rat and 70%-77% with bovine, canine, cotton rat, equine, feline, human, porcine, rat, and rhesus TNFα. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNFα by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNFα. TNFα trimers bind the ubiquitous TNF RI and the hematopoietic cell restricted TNF RII, both of which are also expressed as homotrimers. TNFα regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNFα is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.
Synonyms
Tnf; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha
NCBI Gene ID
21926
UniProt
P06804
Accession Number
NP_038721.1
Accession Number mRNA
NM_013693.2
Chromosomal Location
17 B1; 17 19.06 cM
Reactivity
Anti-Mouse
Cross Reactivity
Mouse
Target Antigen
Recombinant mouse TNF-alpha
Clone
Rabbit IgG
Applications
WB
Purification Method
Protein A purified
Assay Protocol
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Bioactivity
Western Blot: use at 2-5 µg/ml.
Form
Lyophilized
Buffer
PBS
Reconstitution
Water
Storage Conditions
The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
Host or Source
Rabbit

Related Products

CatalogName
600-179-L100TNF-beta / TNFSF1 / Lymphotoxin alpha Protein
600-179-L1000TNF-beta / TNFSF1 / Lymphotoxin alpha Protein
600-179-L20TNF-beta / TNFSF1 / Lymphotoxin alpha Protein
100-108-L500TNF-beta
100-108STNF-beta
100-108-L1000TNF-beta
100-108-L250TNF-beta
100-108-L50TNF-beta
102-P88TNF-beta
100-108TNF-beta

Alternative Products

CatalogName
TS406983P5TNF alpha (TNF) CytoSection
377630TNF alpha
337438-01TNF-alpha

Popular Products