TNF-alpha Recombinant Protein

• Description:

  TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant rat TNF-α is a soluble 157 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

• Synonyms:

  Tnf; Tnfa; RATTNF; TNF-alpha

• CAS Number:

  9000-83-3

• NCBI Gene ID:

  24835

• UniProt:

  P16599

• Accession Number:

  NP_036807

• Accession Number mRNA:

  NM_012675

• Gene Location:

  20p12

• Host:

  E. coli

• Origin Species:

  Rat

• Species Reactivity:

  Mouse, Rat, Bacteria, Human, Monkey

• Sequence:

  MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL

• Detection Range:

  The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Length:

  157

• Form:

  Lyophilized

• Molecular Weight:

  17.3 kDa

• Shipping Conditions:

  Room Temperature