TNF-alpha Recombinant Protein

TNF-alpha Recombinant Protein

• Description: TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant rat TNF-α is a soluble 157 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
• Synonyms: Tnf; Tnfa; RATTNF; TNF-alpha
• CAS Number: 9000-83-3
• NCBI Gene ID: 24835
• UniProt: P16599
• Accession Number: NP_036807
• Accession Number mRNA: NM_012675
• Gene Location: 20p12
• Host: E. coli
• Origin Species: Rat
• Species Reactivity: Mouse, Rat, Bacteria, Human, Monkey
• Sequence: MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
• Detection Range: The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Length: 157
• Form: Lyophilized
• Molecular Weight: 17.3 kDa
• Shipping Conditions: Room Temperature