Rabbit Anti-Mouse TNF-alpha Polyclonal Antibody

Rabbit Anti-Mouse TNF-alpha Polyclonal Antibody

• Description: TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant murine TNF-α is a soluble 156 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
• Synonyms: Tnf; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha
• CAS Number: 9007-83-4
• NCBI Gene ID: 21926
• UniProt: P06804
• Accession Number: NP_038721.1
• Accession Number mRNA: NM_013693.2
• Gene Location: 17 B1; 17 19.06 cM
• Host: Rabbit
• Origin Species: Mouse
• Species Reactivity: Mouse
• Target Antigen: Recombinant Mouse TNF-alpha
• Clone: Rabbit IgG
• Validated Applications: WB
• Purification Method: Protein A purified
• Detection Range: Western Blot: use at 2-5 µg/ml.
• Form: Lyophilized
• Buffer: PBS
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
• Shipping Conditions: Room Temperature
• Storage Conditions: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.