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Podoplanin, soluble

CAT: 0209-S01-046Size: 5 µgDry Ice: NoHazardous: No
CAT#:0209-S01-046Size:5 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid precursor (NP_006465) . It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238) . The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosylation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2) . Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth. The recombinant soluble Podoplanin starts with GLST and ends with GLST.
Synonyms
PDPN; T1A; GP36; GP40; Gp38; OTS8; T1A-2; AGGRUS; HT1A-1; PA2.26
NCBI Gene ID
10630
UniProt
Q86YL7
Accession Number
NP_001006625.1
Accession Number mRNA
NM_001006624.1
Chromosomal Location
1p36.21
Reactivity
Human
Cross Reactivity
Human
Label
His-Tag
Sequence
GSSHHHHHHSSGLVPRGSHMEGASTGQPEDDTETTGLEGVAMPGAEDDVVTPGTSEDRYKSGLTTLVATSVNSVTGIRIEDLPTSESTVHAQEQSPSATASNVATSHsTEKVDGDTQTTVEKDGLST
Assay Protocol
We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
Purity
> 95% by SDS-PAGE
Bioactivity
Testing in progress.
Length
127
Form
Lyophilized
Buffer
PBS
Reconstitution
Water
Molecular Weight
13.1 kDa
Storage Conditions
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted sPodoplanin should be stored in working aliquots at -20°C.
Host or Source
E. coli
N Terminal Sequence
GSHMEGASTGQ

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