Podoplanin

• Description:

  Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. It was originally discovered in kidney podocytes. This mouse monoclonal antibody (IgG) against rat podoplanin works well for immunohistochemistry with formalin-fixed paraffin embedded sections. This antibody also can be used in FACS sorting.

• Synonyms:

  Pdpn; E11; Gp38; OTS-8; RTI40; T1-alpha

• NCBI Gene ID:

  54320

• UniProt:

  Q64294

• Accession Number:

  NP_062231.1

• Accession Number mRNA:

  NM_019358.1

• Chromosomal Location:

  5q36

• Reactivity:

  Anti-Rat

• Cross Reactivity:

  Rat

• Target Antigen:

  Membrane protein fraction of isolated rat glomeruli

• Clone:

  (#LF3 (B7) D5B3)

• Applications:

  WB, IHC

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  Western Blot: use at 1-5 µg/ml

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source:

  Mouse

• Isotype:

  IgG