Podoplanin

• Description:

  Podoplanin, also known as T1 alpha (T1α), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

• Synonyms:

  Pdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha

• NCBI Gene ID:

  14726

• UniProt:

  Q62011

• Accession Number:

  NP_034459.2

• Accession Number mRNA:

  NM_010329.2

• Chromosomal Location:

  4 E1; 4

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Murine stromal cell line

• Clone:

  (#RTD4E10)

• Applications:

  WB, IHC, IP

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  Western Blot: use at 1-5 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source:

  Hamster

• Isotype:

  IgG1