PDGFR-alpha

• Description:

  The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGFA, B, C, and D) that form disulfide-linked homodimers (PDGFAA, BB, CC, and DD) and a heterodimer (PDGFAB) . These proteins regulate diverse cellular functions by binding to and inducing the homoor heterodimerization of two receptors (PDGF Rα and Rβ) . Whereas α/α homodimerization is induced by PDGF-AA, BB, CC, and AB, α/β heterodimerization is induced by PDGF-AB, BB, CC, and DD, and β/β homodimerization is induced only by PDGF-BB and DD. Both PDGF Rα and Rβ are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for MCSF, SCF, and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulinlike domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-iinduced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the α and β receptors. Others have only the α receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the β receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) . Recombinant mouse and human soluble PDGF Rβ bind PDGF with high affinity and are potent PDGF antagonists.

• Synonyms:

  Pdgfra; CD140a; Pdgfr-2; AI115593

• NCBI Gene ID:

  18595

• UniProt:

  P26618

• Accession Number:

  NP_001076785.1

• Accession Number mRNA:

  NM_001083316.1

• Chromosomal Location:

  5 C3.3; 5 42.0 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Recombinant mouse PDGFR alpha extracellular domain

• Clone:

  (#3G28)

• Applications:

  WB, IHC (P)

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Bioactivity:

  WB: 1:400-1000; IHC: 1:50-800

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG2