PDGFR-alpha

CAT: 0209-103-M43Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0209-103-M43Size:100 µg

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Description

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGFA, B, C, and D) that form disulfide-linked homodimers (PDGFAA, BB, CC, and DD) and a heterodimer (PDGFAB) . These proteins regulate diverse cellular functions by binding to and inducing the homoor heterodimerization of two receptors (PDGF Rα and Rβ) . Whereas α/α homodimerization is induced by PDGF-AA, BB, CC, and AB, α/β heterodimerization is induced by PDGF-AB, BB, CC, and DD, and β/β homodimerization is induced only by PDGF-BB and DD. Both PDGF Rα and Rβ are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for MCSF, SCF, and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulinlike domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-iinduced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the α and β receptors. Others have only the α receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the β receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) . Recombinant mouse and human soluble PDGF Rβ bind PDGF with high affinity and are potent PDGF antagonists.

Synonyms

Pdgfra; CD140a; Pdgfr-2; AI115593

NCBI Gene ID

18595

UniProt

P26618

Accession Number

NP_001076785.1

Accession Number mRNA

NM_001083316.1

Chromosomal Location

5 C3.3; 5 42.0 cM

Reactivity

Anti-Mouse

Cross Reactivity

Mouse

Target Antigen

Recombinant mouse PDGFR alpha extracellular domain

Clone

(#3G28)

Applications

WB, IHC (P)

Purification Method

Protein G chromatography

Assay Protocol

Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Bioactivity

WB: 1:400-1000; IHC: 1:50-800

Form

Lyophilized

Buffer

PBS

Reconstitution

PBS

Storage Conditions

Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

Host or Source

Rat

Isotype

IgG2

UniProtKB · P26618

Platelet-derived growth factor receptor alpha

PGFRA_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: P26618
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Pdgfra
Protein existence: 1: Evidence at protein level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: PDGF-R-alpha, PDGFR-alpha
EC number: 2.7.10.1
Processing: Precursor
Secondary accessions: Q3TQ37, Q62046, Q7TSJ3, Q8C4N3

Protein keywords

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell membraneCell projectionGolgi apparatusMembrane

Biological process

Chemotaxis

Molecular function

Developmental proteinKinaseReceptorTransferaseTyrosine-protein kinase

PTM

Disulfide bondGlycoproteinPhosphoproteinUbl conjugation

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

Disease

Proto-oncogene

Technical term

Reference proteome

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Alternative Products

Catalog	Name
526802-01	PDGFR alpha
MBS692682-01	PDGFR-alpha
70R-30719	PDGFR alpha antibody
540838-01	PDGFR-alpha discontinued