Lyve-1, soluble

CAT: 0209-S01-028Size: 20 µgDry Ice: NoHazardous: No
CAT#:0209-S01-028Size:20 µg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
A DNA sequence encoding the extracellular domain of human LYVE-1 (Met1 to Gly232) was fused to a C-terminal His-tag (6xHis) and expressed in insect cells. Based on N-terminal sequence analysis, the primary structure of recombinant mature sLYVE-1 starts at Ser24. sLYVE-1 has a calculated monomeric molecular mass of about 25 kDa but as a result of glycosylation, migrates at approximately 35 - 45 kDa under reducing conditions in SDS-PAGE. LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Synonyms
LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
NCBI Gene ID
10894
UniProt
Q9Y5Y7
Accession Number
NP_006682
Accession Number mRNA
NM_006691
Chromosomal Location
11p15
Reactivity
Human
Cross Reactivity
Human
Label
His-Tag
Sequence
SLRAEELSIQVSCRIMGITLVSKKANQQLNFTEAKEACRLLGLSLAGKDQVETALKASFETCSYGWVGDGFVVISRISPNPKCGKNGVGVLIWKVPVSRQFAAYCYNSSDTWTNSCIPEIITTKDPIFNTQTATQTTEFIVSDSTYSVASPYSTIPAPTTTPPAPASTSIPRRKKLICVTEVFMETSTMSTETEPFVENKAAFKNEAAGHHHHHH
Assay Protocol
The lyophilized sLYVE-1 is soluble in water and most aqueous buffers. The lyophilized sLYVE-1 should be reconstituted in PBS or medium to a concentration not lower than 50µg/ml.
Purity
> 95% by SDS-PAGE
Bioactivity
Not tested so far!
Length
215
Form
Lyophilized
Buffer
PBS
Reconstitution
Water
Molecular Weight
~ 35.0 - 45.0 kDa
Storage Conditions
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sLYVE-1 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
Host or Source
Insect cells
N Terminal Sequence
SLRAEELSI

Related Products

CatalogName
102-PA50Lyve-1
103-PA50AGLyve-1
103-PABi50Lyve-1
103-M130Lyve-1
102-PA50SLyve-1
103-PA50Lyve-1
103-PA50SLyve-1
102-PA50AGLyve-1
101-M130Lyve-1
102-PABi50Lyve-1

Alternative Products

CatalogName
MBS691767-01Human Lyve-1, soluble
MBS692061-01Mouse Lyve-1, soluble
MBS692589Desmoglein-1, soluble
304443Aconitase 1, soluble
MBS692170-01Human DLL-1, soluble
550163Thymidine Kinase 1, Soluble
333776Serine hydroxymethyltransferase 1 (soluble)
MBS692283-01Human FRP-1, soluble
MBS692158-01Mouse TIE-1, soluble
548234Isocitrate Dehydrogenase 1, Soluble

Popular Products