Lyve-1, soluble

• Description :

  A DNA sequence encoding the extracellular domain of human LYVE-1 (Met1 to Gly232) was fused to a C-terminal His-tag (6xHis) and expressed in insect cells. Based on N-terminal sequence analysis, the primary structure of recombinant mature sLYVE-1 starts at Ser24. sLYVE-1 has a calculated monomeric molecular mass of about 25 kDa but as a result of glycosylation, migrates at approximately 35 - 45 kDa under reducing conditions in SDS-PAGE. LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

• Synonyms :

  LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1

• NCBI Gene ID :

  10894

• UniProt :

  Q9Y5Y7

• Accession Number :

  NP_006682

• Accession Number mRNA :

  NM_006691

• Chromosomal Location :

  11p15

• Reactivity :

  Human

• Cross Reactivity :

  Human

• Label :

  His-Tag

• Sequence :

  SLRAEELSIQVSCRIMGITLVSKKANQQLNFTEAKEACRLLGLSLAGKDQVETALKASFETCSYGWVGDGFVVISRISPNPKCGKNGVGVLIWKVPVSRQFAAYCYNSSDTWTNSCIPEIITTKDPIFNTQTATQTTEFIVSDSTYSVASPYSTIPAPTTTPPAPASTSIPRRKKLICVTEVFMETSTMSTETEPFVENKAAFKNEAAGHHHHHH

• Assay Protocol :

  The lyophilized sLYVE-1 is soluble in water and most aqueous buffers. The lyophilized sLYVE-1 should be reconstituted in PBS or medium to a concentration not lower than 50µg/ml.

• Purity :

  > 95% by SDS-PAGE

• Bioactivity :

  Not tested so far!

• Length :

  215

• Form :

  Lyophilized

• Buffer :

  PBS

• Reconstitution :

  Water

• Molecular Weight :

  ~ 35.0 - 45.0 kDa

• Storage Conditions :

  Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sLYVE-1 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

• Host or Source :

  Insect cells

• N Terminal Sequence :

  SLRAEELSI