IL-10 Recombinant Protein

IL-10 Recombinant Protein

• Description: Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of mouse T helper (Th) 2 clones that inhibited cytokine production by Th1 clones . The human ortholog of mouse IL-10 was subsequently cloned by cross-hybridization. Human and mouse IL-10 are 81% identical at the nucleotide and amino acid level. IL-10 is the prototypic member of the IL-10 cytokine family, including IL-10, IL-19, IL-20, IL-22 (IL-TIF), IL-24 and IL-26. A number of viruses, including Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV), also encode viral members of the IL-10 family. Human IL-10 has cross-species actvity and is active on mouse cells. Mouse IL-10 is species-specific and does not act on human cells.
• Synonyms: Il10; CSIF; Il-10
• CAS Number: 9000-83-3
• NCBI Gene ID: 16153
• UniProt: P18893
• Accession Number: NP_034678
• Accession Number mRNA: NM_010548
• Gene Location: 1 E4; 1 69.9 cM
• Host: E. coli
• Origin Species: Mouse
• Species Reactivity: Human, Rat, Mouse
• Sequence: MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S
• Detection Range: The biologically activity was measured by the dose-dependent co-stimulation (with IL-4) of the proliferation of murine MC/9 cells. The ED50 was found to be < 2 ng/ml (> 5 x 105 units/mg).
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Length: 161
• Form: Lyophilized
• Molecular Weight: 18.7 kDa
• Shipping Conditions: Room Temperature