IL-6

• Description:

  IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R α and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9 kDa protein containing 184 amino acid residues.

• Synonyms:

  IL6; HGF; HSF; BSF2; IL-6; IFNB2

• NCBI Gene ID:

  3569

• UniProt:

  P05231

• Accession Number:

  NP_000579

• Accession Number mRNA:

  NM_000600

• Chromosomal Location:

  7p21

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Human recombinant IL-6

• Clone:

  (#1X45)

• Applications:

  WB, IHC (F), N/B

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG2