IL-3

• Description:

  IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues.

• Synonyms:

  Il3; BPA; PSF; HCGF; Il-3; MCGF; Csfmu

• NCBI Gene ID:

  16187

• UniProt:

  P01586

• Accession Number:

  NP_034686.2

• Accession Number mRNA:

  NM_010556.4

• Chromosomal Location:

  11 B1; 11 28.5 cM

• Reactivity:

  Mouse

• Cross Reactivity:

  Rat, Mouse, Human, Monkey, Bacteria, Gerbil

• Sequence:

  ASISGRDTHR LTRTLNCSSI VKEIIGKLPE PELKTDDEGP SLRNKSFRRV NLSKFVESQG EVDPEDRYVI KSNLQKLNCC LPTSANDSAL PGVFIRDLDD FRKKLRFYMV HLNDLETVLT SRPPQPASGS VSPNRGTVEC

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & Coomassie stain

• Bioactivity:

  The ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is in the range of 20-100 pg/ml.

• Length:

  140

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Molecular Weight:

  15.8 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  ASISGR