IL-3

• Description:

  IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues.

• Synonyms:

  IL3; IL-3; MCGF; MULTI-CSF

• NCBI Gene ID:

  3562

• UniProt:

  P08700

• Accession Number:

  NP_000579

• Accession Number mRNA:

  NM_000588

• Chromosomal Location:

  5q31.1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  MAPMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF

• Assay Protocol:

  The lyophilized IL-3 should be reconstituted in water to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  >98% by SDS-PAGE

• Bioactivity:

  The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is < 0.1 – 0.5 ng/ml. The WHO standard #91/510 was used as a control.

• Length:

  134

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Molecular Weight:

  15.0 kDa

• Storage Conditions:

  The lyophilized IL-3, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-3 should be stored in working aliquots at -20°C.

• Host or Source:

  E. coli

• N Terminal Sequence:

  MAPMTQT