IL-2

• Description:

  IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant human IL-2 (modified at the C-terminal end) is a 15.5 kDa protein, containing 156 amino acid residues including one intrachain disulfide bond.

• Synonyms:

  IL2; IL-2; TCGF; lymphokine

• NCBI Gene ID:

  3558

• UniProt:

  P60568

• Accession Number:

  NP_000577

• Accession Number mRNA:

  NM_000586

• Chromosomal Location:

  4q26-q27

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLHYKDPAANKARKEAELAAATAEQ

• Assay Protocol:

  The lyophilized IL-2 should be reconstituted in 50mM acetic acid to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.

• Endotoxin:

  < 0.1 ng per µg of IL-2

• Purity:

  > 98% by SDS-PAGE

• Bioactivity:

  The ED50 as determined by the dose-dependent stimulation of proliferation of murine D10G4.1 cells is in the range of 0.1-0.5 ng/ml.

• Length:

  156

• Form:

  Lyophilized

• Buffer:

  50mM acetic acid

• Reconstitution:

  50mM acetic acid

• Molecular Weight:

  15.5 kDa

• Storage Conditions:

  The lyophilized IL-2, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-2 should be stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.

• Host or Source:

  E. coli

• N Terminal Sequence:

  APTSSSTK and MAPTSSST