IFN gamma

• Description:

  IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ is species-specific and is biologically active only in human and primate cells. Recombinant rat IFN-γ is a 15.6 kDa protein containing 135 amino acid residues.

• Synonyms:

  Ifng; IFNG2

• NCBI Gene ID:

  25712

• UniProt:

  P01581

• Accession Number:

  NP_620235.1

• Accession Number mRNA:

  NM_138880.2

• Chromosomal Location:

  7q22

• Reactivity:

  Rat

• Cross Reactivity:

  Mouse, Rat, Human

• Sequence:

  MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 as determined by a cytopathic affect inhibition assay with murine L929 cells challenged with EMC virus was ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

• Length:

  135

• Form:

  Lyophilized

• Molecular Weight:

  15.6 kDa

• Host or Source:

  E. coli