IFN gamma Recombinant Protein

• Description:

  Interferon-gamma (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Recombinant Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.

• Synonyms:

  IFNG; IFG; IFI

• NCBI Gene ID:

  3458

• UniProt:

  P01579

• Accession Number:

  NP_000610.2

• Accession Number mRNA:

  NM_000619

• Gene Location:

  12q14

• Host:

  E. coli

• Origin Species:

  Human

• Species Reactivity:

  Monkey, Human, Mouse, Leech, Pig,

• Sequence:

  MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

• Detection Range:

  Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml. Assay #2: The ED50 was determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml (> 2 x 107 units/mg).

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Length:

  144

• Form:

  Lyophilized

• Buffer:

  10mM Sodium Phosphate, pH 7.4

• Reconstitution:

  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to ‑80°C.

• Molecular Weight:

  16.8 kDa

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.

• CAS Number:

  9000-83-3