Heregulin beta-1 (Animal Free) Recombinant Protein

Heregulin beta-1 (Animal Free) Recombinant Protein

• Description: Neuregulin/Heregulin is a family of structurally-related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms releases soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain; the latter is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity that leads to tyrosine phosphorylation. Although HRG1-β1's biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells, which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). Recombinant Human Heregulinβ-1 (HRG1-B1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulinβ-1 (65 amino acid residues).
• Synonyms: NRG1; GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131
• CAS Number: 9000-83-3
• NCBI Gene ID: 3084
• UniProt: Q02297
• Accession Number: NP_039258.1
• Accession Number mRNA: NM_013964.3
• Gene Location: 8p12
• Host: E. coli
• Origin Species: Human
• Species Reactivity: Human, Mouse, Rat
• Sequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE
• Detection Range: The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106 units/mg.
• Endotoxin: < 0.01 ng/ug of protein (< 0.1 EU/ug)
• Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
• Length: 65
• Form: Lyophilized
• Molecular Weight: 7.5 kDa
• Shipping Conditions: Room Temperature