GM-CSF

• Description:

  GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM-CSF, similar to IL3 and IL5, is a cytokine with a core of four bundled α-helices. Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF Rα/CD116 and the signal transducing common β chain (CD131) which is also a component of the high affinity receptors for IL-3 and IL-5. In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF Rα. The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells.

• Synonyms:

  Csf2, Csfgm; GM-CSF; Gm-CSf; MGI-IGM

• NCBI Gene ID:

  12981

• UniProt:

  P01587

• Accession Number:

  NP_034099.2

• Accession Number mRNA:

  NM_009969.4

• Chromosomal Location:

  11 B1.3; 11 29.5 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Recombinant mouse GM-CSF (RT #M30-013)

• Clone:

  Rabbit IgG

• Applications:

  WB

• Purification Method:

  Protein A purified

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  Western Blot: use at 2-5 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source:

  Rabbit