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GM-CSF

CAT: 0209-M30-012Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0209-M30-012Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant murine GM-CSF is a 14.2 kDa globular protein consisting of 124 amino acids residues.
Synonyms
Csf2; Csfgm; GMCSF; Gm-CSf; MGI-IGM
NCBI Gene ID
12981
UniProt
P01587
Accession Number
NP_034099.2
Accession Number mRNA
NM_009969.4
Chromosomal Location
11 B1.3; 11 29.5 cM
Reactivity
Mouse
Cross Reactivity
Mouse
Sequence
MAPTRSPITVTRPWKHVEAIKEALNLLDDMPVTLNEEVEVVSNEFSFKKLTCVQTRLKIFEQGLRGNFTKLKGALNMTASYYQTYCPPTPETDCETQVTTYADFIDSLKTFLTDIPFECKKPGQK
Assay Protocol
The lyophilized mouse GM-CSF is soluble in water and most aqueous buffers. It can be reconstituted in water to a concentration of 100 µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use. For most in vitro applications, mouse GM-CSF exerts its biological activity in the concentration range of 0.05 to 0.5ng/ml.
Endotoxin
< 0.1 ng per µg of GM-CSF
Purity
> 98% by SDS-PAGE
Bioactivity
Testing in Progress.
Length
125
Form
Lyophilized
Buffer
30 mM Tris pH8
Reconstitution
Water
Molecular Weight
14.2 kDa
Storage Conditions
The lyophilized mouse GM-CSF, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted mouse GM-CSF should be stored in working aliquots at -20°C.
Host or Source
E. coli
N Terminal Sequence
MAPTRSPITV

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