Flt-3 Ligand Recombinant Protein

• Description:

  Flt3 Ligand, also known as FL, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature mouse Flt3 Ligand consists of a 161 amino acid (aa) extracellular domain (ECD) with a cytokinelike domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 22 aa cytoplasmic tail. Within the ECD, mouse Flt3 Ligand shares 71% and 81% aa sequence identity with human and rat Flt3 Ligand, respectively. Mouse and human Flt3 Ligand show cross-species activity. Flt3 Ligand is expressed as a non-covalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of mouse Flt3 Ligand also generates a membrane-associated isoform with a 57 aa substitution following the cytokine domain. Both transmembrane and soluble Flt3 Ligand signal through the tyrosine kinase receptor Flt3/ Flk2.

• Synonyms:

  Flt3; Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04

• NCBI Gene ID:

  14255

• UniProt:

  P49772

• Accession Number:

  NP_038548.3

• Accession Number mRNA:

  NM_013520.3

• Gene Location:

  5 G3; 5 82.0 cM

• Host:

  E. coli

• Origin Species:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Sequence:

  MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ

• Detection Range:

  Determined by the dose-dependent stimulation of the proliferation of human AML5 cells. The expected ED50 for this effect is 5.0 – 8.0 ng/ml.

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Length:

  163

• Form:

  Lyophilized

• Molecular Weight:

  18.6 kDa

• Shipping Conditions:

  Room Temperature

• CAS Number:

  9000-83-3