FGF-10

• Description :

  FGF-10 is a heparin-binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-10 is most related to FGF-7/KGF, and is expressed during the development and, preferentially, in adult lungs. It signals through FGFR 2b. Recombinant Murine FGF-10 is a 17.0 kDa protein consisting of 148 amino acid residues.

• Synonyms :

  Fibroblast Growth Factor‐10, FGFA, Keratinocyte Growth Factor‐2

• NCBI Gene ID :

  14165

• UniProt :

  O35565

• Accession Number :

  NP_032028.1

• Accession Number mRNA :

  NM_008002.4

• Reactivity :

  Mouse

• Cross Reactivity :

  Mouse

• Sequence :

  SSAGRHVRSY NHLQGDVRWR RLFSFTKYFL TIEKNGKVSG TKNEDCPYSV LEITSVEIGV VAVKAINSNY YLAMNKKGKL YGSKEFNNDC KLKERIEENG YNTYASFNWQ HNGRQMYVAL NGKGAPRRGQ KTRRKNTSAH FLPMTIQT

• Assay Protocol :

  Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  ≥ 95% by SDS-PAGE gel and HPLC analyses

• Bioactivity :

  Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8) .

• Length :

  148

• Form :

  Lyophilized

• Reconstitution :

  Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Molecular Weight :

  17.0 kDa

• Storage Conditions :

  The lyophilized protein is stable at room temperature for 3 weeks should be stored desiccated below -18°C. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

• Host or Source :

  E. coli