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FGF-7/KGF

CAT: 0209-M10-042Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0209-M10-042Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
FGF‐7/KGF is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth, and the regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF‐7/KGF is a mitogen factor specific for epithelial cells and keratinocytes. FGF‐7/KGF signals through FGFR 2b. FGF‐7/KGF plays a role in kidney and lung development, as well as in angiogenesis and wound healing. Recombinant Murine FGF‐7/KGF is an 18.9 kDa protein consisting of 164 amino acid residues.
Synonyms
Keratinocyte Growth Factor, Fibroblast Growth Factor-7, HBGF-7
NCBI Gene ID
14178
UniProt
P36363
Accession Number
NP_032034.1
Accession Number mRNA
NM_008008.4
Reactivity
Mouse
Cross Reactivity
Mouse
Sequence
MCNDMSPEQT ATSVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNSYNIMEI RTVAVGIVAI KGVESEYYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHSG GEMFVALNQK GIPVKGKKTK KEQKTAHFLP MAIT
Assay Protocol
Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
≥ 95% by SDS-PAGE gel and HPLC analyses
Bioactivity
Determined by a cell proliferation assay using 4MBr-5 cells. The expected ED50 for this effect is 0.5-5.0 ng/ml.
Length
164
Form
Lyophilized
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Molecular Weight
18.9 kDa
Storage Conditions
The lyophilized protein is stable at room temperature for 3 weeks should be stored desiccated below -18°C. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
Host or Source
E. coli

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