FGF-2 (basic)

CAT: 0209-B30-003Size: 10 µgDry Ice: NoHazardous: No
CAT#:0209-B30-003Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
FGF2 (basic) is one of at least 23 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. FGF2 has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum and placenta. This factor contains four cysteine residues but reduced FGF2 retains full biological activity, indicating that disulfide bonds are not required for this activity. Several reports indicate that a variety of forms of FGF2 are produced as a result of N-terminal extensions. These extensions apparently affect localization of FGF2 in cellular compartments but do not affect biological activity. Studies indicate that binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF2 stimulates the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin. The cells include fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, osteoblasts, smooth muscle cells, and melanocytes. FGF2 is chemotactic and mitogenic for endothelial cells in vitro. FGF2 induces neuron differentiation, survival and regeneration. The 17 kDa bovine sequence has 95% aa identity with human and sheep FGF basic.
Synonyms
Fgf2; bFGF; Fgf-2
NCBI Gene ID
281161
UniProt
P03969
Accession Number
NP_776481.2
Accession Number mRNA
NM_174056
Reactivity
Bovine
Cross Reactivity
Bovine
Sequence
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGPKTGP GQKAILFLPM SAKS
Assay Protocol
The bovine FGF-2 is supplied in lyophilized form and can be reconstituted with ddH2O at 50 µg/mL. This solution can be diluted into other buffered solutions or stored frozen for future use. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.
Endotoxin
< 0.1 ng per µg of bovine FGF-2
Purity
> 98% by SDS-PAGE
Bioactivity
The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells (HUVEC) by bovine FGF-2 has been determined to be in the range of 0.1-2 ng/ml.
Length
145
Form
Lyophilized
Buffer
0.5X PBS
Reconstitution
Water
Molecular Weight
16.34 kDa
Storage Conditions
The lyophilized bovine FGF-2, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C
Host or Source
E. coli

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