FGF acidic Recombinant Protein

FGF acidic Recombinant Protein

• Description: FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant mouse FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues.
• Synonyms: HBGF-1; aFGF; acidic fibroblast growth factor; fibroblast growth factor 1 (acidic); heparin-binding growth factor 1
• CAS Number: 9000-83-3
• NCBI Gene ID: 14164
• UniProt: P61148
• Accession Number: NP_034327.1
• Accession Number mRNA: NM_010197.3
• Gene Location: 18 19.0 cM
• Host: E. coli
• Origin Species: Mouse
• Species Reactivity: Mouse
• Sequence: MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
• Detection Range: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is ≤ 0.5 ng/ml (≥ 2 x 106 units/mg). Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.2 ng/ml in the presence of 10 μg/ml heparin (≥ 5 x 106 units/mg)
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Length: 141
• Form: Lyophilized
• Molecular Weight: 15.9 kDa
• Shipping Conditions: Room Temperature