FGF acidic Recombinant Protein

FGF acidic Recombinant Protein

• Description: FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGF-acidic is a 15.8 kDa protein consisting of 140 amino acid residues.
• Synonyms: FGF2; BFGF; FGFB; HBGF-2
• CAS Number: 9000-83-3
• NCBI Gene ID: 2246
• UniProt: P05230
• Accession Number: NP_000791.1
• Accession Number mRNA: NM_000800.4
• Gene Location: 5q31
• Host: E. coli
• Origin Species: Human
• Species Reactivity: Cow, Mouse, PIG, Rat, Human, Hamster, Leech
• Sequence: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
• Detection Range: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/ml. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.5 ng/ml in the presence of 10 μg/ml heparin.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 95% by SDS-PAGE & HPLC analyses
• Length: 141
• Form: Lyophilized
• Buffer: 5mM Sodium Phosphate, pH 8.0 + 100mM NaCl
• Molecular Weight: 15.8 kDa
• Shipping Conditions: Room Temperature