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FGF acidic

CAT: 0209-R20-018Size: 50 µgDry Ice: NoHazardous: No
CAT#:0209-R20-018Size:50 µg
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Description
FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant rat FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues.
Synonyms
Fgf1; FGF-1; HBGF1; HBGF-1
NCBI Gene ID
25317
UniProt
P61149
Accession Number
NP_036978.1
Accession Number mRNA
NM_012846.1
Chromosomal Location
18p11
Reactivity
Rat
Cross Reactivity
Rat
Sequence
MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Assay Protocol
Reconstitute in 5 mM Sodium Phosphate, pH 7.2 to a concentration of ≤ 0.5 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98% by SDS-PAGE & HPLC analyses
Bioactivity
Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is ≤ 0.1 ng/ml, in the presence of 10 µg/ml heparin, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Length
141
Form
Lyophilized
Buffer
10mM Sodium Phosphate, pH 7.2 + 150mM NaCl + 0.2% Chaps
Reconstitution
5mM Sodium Phosphate, pH 7.2
Molecular Weight
15.9 kDa
Storage Conditions
The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
Host or Source
E. coli

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