FGF acidic

• Description:

  FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant mouse FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues.

• Synonyms:

  HBGF-1; aFGF; acidic fibroblast growth factor; fibroblast growth factor 1 (acidic) ; heparin-binding growth factor 1

• NCBI Gene ID:

  14164

• UniProt:

  P61148

• Accession Number:

  NP_034327.1

• Accession Number mRNA:

  NM_010197.3

• Chromosomal Location:

  18 19.0 cM

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Sequence:

  MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is ≤ 0.5 ng/ml (≥ 2 x 106 units/mg) . Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.2 ng/ml in the presence of 10 μg/ml heparin (≥ 5 x 106 units/mg)

• Length:

  141

• Form:

  Lyophilized

• Molecular Weight:

  15.9 kDa

• Host or Source:

  E. coli