E-Cadherin

• Description:

  Epithelial (E) Cadherin (E-CAD), also known as Cadherin1, cell-CAM120/ 80 in the human, uvomorulin in the mouse, Arc1 in the dog, and LCAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1) . Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, α, β, and γ (plakoglobin) . This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a singlepass-transmembrane domain, and a short carboxyterminal cytoplasmic domain responsible for interacting with the catenins. E-Cadherin contains five extracellular calciumbinding domains of approx. 110 amino acids each (amino acids 155-697) .

• Synonyms:

  CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324

• NCBI Gene ID:

  999

• UniProt:

  P12830

• Accession Number:

  NP_004351.1

• Accession Number mRNA:

  NM_004360.3

• Chromosomal Location:

  16q22.1

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human E-Cadherin protein

• Clone:

  (#21E20)

• Applications:

  WB

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG2