E-Cadherin

• Description:

  E-Cadherin/Cadherin1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion. Mouse ECadherin shares 92% and 81% amino acid sequence identity with the rat and human proteins, respectively. It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier.

• Synonyms:

  Cadherin-1; ARC-1, Epithelial cadherin (E-cadherin), Uvomorulin

• NCBI Gene ID:

  12550

• UniProt:

  P09803

• Accession Number:

  NP_033994.1.

• Accession Number mRNA:

  NM_009864.3

• Reactivity:

  Anti-Mouse

• Target Antigen:

  Mouse recombinant protein E-cadherin

• Clone:

  (#6N32)

• Applications:

  E

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG1