CD25

• Description:

  The biological effects of interleukin 2 (IL-2) are mediated by its binding to a multi-chain cellular receptor complex. The IL-2 receptor (IL-2 R) complex consists of three glycoprotein chains. The alpha chain (IL-2 R alpha /CD25) and beta chain (IL-2 R beta /CD122) act together with the gamma chain (IL-2 R gamma /CD132) to form a high affinity receptor that transduces the signal upon IL-2 binding. Alone, IL-2 R alpha binds IL-2 with low affinity, but is unable to transduce a signal. The alpha/beta combination binds IL-2 with intermediate affinity, but is still unable to transmit a signal inside the cell. The beta/gamma complex has intermediate affinity and is capable of signaling if the concentration of IL-2 is relatively high.

• Synonyms:

  CD25; Il2r; Ly-43; interleukin 2 receptor, alpha chain; Il2ra

• NCBI Gene ID:

  16184

• UniProt:

  P01590

• Accession Number:

  NP_032393.3

• Accession Number mRNA:

  NM_008367.3

• Chromosomal Location:

  2 A2-A3; 2 6.4 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Recombinant Mouse CD25 protein

• Clone:

  (#MAB0710)

• Applications:

  IHC, FC

• Purification Method:

  Protein G/A chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS (sterile)

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG1