Cathepsin L

• Description:

  Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinasetype plasminogen activator, which is activated by Cathepsin L cleavage. Cathepsin L has also been shown to proteolytically inactivate α1antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract. These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth. In Cathepsin L deficient mice, it appears to play a critical role in cardiac morphology and function, epidermal homeostasis, regulation of the hair cycle, and MHC class I-mediated antigen presentation in cortical epithelial cells of the thymus. Mouse Cathepsin L is synthesized as a 334 amino acid precursor with a signal peptide (residues 117), a pro region (residues 18-113), and a mature chain (residues 114-334) .

• Synonyms:

  Ctsl; fs; MEP; nkt; Ctsl1; 1190035F06Rik

• NCBI Gene ID:

  13039

• UniProt:

  P06797

• Accession Number:

  NP_034114

• Accession Number mRNA:

  NM_009984

• Chromosomal Location:

  13 B3; 13 30.0 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Recombinant mouse Cathepsin-L

• Clone:

  (#5G14)

• Applications:

  WB, IHC

• Purification Method:

  Protein G/A chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG2