Cathepsin D

• Description:

  Cathepsin D is a lysosomal aspartic protease of the pepsin family (1) . Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (aa 1-18), a propeptide (aa 19-64), and a mature chain (aa 65-412) ) . The mature chain can be processed further to the light (aa 65-161) and heavy (aa 169-412) chains. It is expressed in most cells and overexpressed in breast cancer cells. It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis. Cathepsin D secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis.

• Synonyms:

  CTSD; CPSD; CLN10

• NCBI Gene ID:

  1509

• UniProt:

  P07339

• Accession Number:

  NP_001900

• Accession Number mRNA:

  NM_001909

• Chromosomal Location:

  11p15.5

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human Cathepsin-D

• Clone:

  (#13P35)

• Applications:

  Detection Ab

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG1