BAFF (Animal Free)

• Description:

  BAFF, a member of the TNF family of ligands, is expressed in T cells, macrophages, monocytes and dendritic cells. BAFF is involved in stimulation of B and T cell function, and is an important survival and maturation factor for peripheral B cells. BAFF signals through three different TNF receptors, TACI, BCMA and BAFF-R. The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain. Recombinant Human soluble BAFF is a 152 amino acid polypeptide (17.0 kDa), which contains the TNF-like portion of the extracellular domain of BAFF.

• Synonyms:

  TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF22

• NCBI Gene ID:

  10673

• UniProt:

  Q9Y275

• Accession Number:

  NP_006564.1

• Accession Number mRNA:

  NM_006573.4

• Chromosomal Location:

  13q32-q34

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

• Endotoxin:

  < 0.01 ng/µg of protein (< 0.1 EU/µg)

• Purity:

  ≥ 95% by SDS-PAGE gel and HPLC analyses.

• Bioactivity:

  Determined by a mouse splenocyte survival assay. The expected ED50 for this effect is 0.5-2.0 µg/ml.

• Length:

  153

• Form:

  Lyophilized

• Molecular Weight:

  17.0 kDa

• Host or Source:

  E. coli