Recombinant Human Interferon-alpha1b

• Background:

  IFN-αs are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-α family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

• Gene ID:

  3439

• UniProt:

  P01562

• Target:

  MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNVDSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE

• Purification:

  > 96 % by SDS-PAGE and HPLC analyses.

• Endotoxin:

  Less than 1 EU/ug of rHuIFN-α1b as determined by LAL method.

• Bioactivity:

  8

• Storage Conditions:

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

• Calculated Molecular Weight:

  Approximately 19.5 kDa, a single non-glycosylated polypeptide chain containing 167 amino acids.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4, containing 4 % mannitol and 1 % HSA.

• Host or Source:

  Escherichia coli