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Recombinant Human Interferon-alpha4a, Yeast

CAT: 0355-AP60101Size: InquireDry Ice: NoHazardous: No
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CAT#:0355-AP60101Size:Inquire
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are mainly involved in innate immune response against viral infection. The IFN- alpha; family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN- alpha; subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Interferon-alpha 4 (IFNA4) belongs to the alpha interferon family. IFNA4 is a secreted protein and produced by macrophages. Two variants of IFNA4 (IFNA4a and IFNA4b) are known, which differ from each other by changes in their coding regions at nucleotide positions 220 and 410.
Target
CDLPQTHSLG NRRALILLAQ MGRISHFSCL KDRHDFGFPE EEFDGHQFQK AQAISVLHEM IQQTFNLFST EDSSAAWEQS LLEKFSTELY QQLNDLEACV IQEVGVEETP LMNEDSILAV RKYFQRITLY LTEKKYSPCA WEVVRAEIMR SLSFSTNLQK RLRRKD
Purification
> 95 % by SDS-PAGE analyses.
Endotoxin
Less than 1 EU/ug of rHuIFN-α4a, Yeast as determined by LAL method.
Bioactivity
Test in process.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculated Molecular Weight
Approximately 19.4 kDa, a single polypeptide chain containing 166 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.0.
Host or Source
Yeast

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