Steady-Luc™ Firefly HTS Assay Kit, 1000 Assays
CAT:
37-30028-2
Size:
1 Kit
Price:
Ask
- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: Yes




Steady-Luc™ Firefly HTS Assay Kit, 1000 Assays
- Description: Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples. Biotium's Steady-Luc trade; HTS assay system is a proprietary mixture of substances that modify the enzymatic reaction to produce a long lasting signal (steady glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a homogeneous high sensitivity firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates. Glow-type luciferase assays like Steady-Luc trade; have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity. Biotium also offers Steady-Luc trade; Firefly HTS Assay Kit (Lyophilized), with lyophilized assay buffer for convenient room temperature shipping and storage at -20°C.
Kit Components:
- D-Luciferin
- Steady-Luc Firefly Assay Buffer
- Simple: single step homogeneous assay.
- Glow kinetics: luminescence half life of about 3 hours.
- Economic: save up to 45% compared to other brands.
- UNSPSC: 41106513
- UNSPSC Description: Reporter gene assay
- Source: Synthetic
- Storage Conditions: <-60°C
- Shelf Life: 6 months