Firefly Luciferase Assay Kit

• Description:

  Luciferase-based assays are the most sensitive assays for studying gene regulation and function. Firefly luciferase is 100-fold more sensitive than the alternative reporters including chloramphenicol acetyltransferase, β-Glucuronidase and green fluorescent protein assays. BioVision’s Firefly Luciferase Assay Kit is designed for an accurate, sensitive and efficient quantitation of firefly luciferase reporter enzyme activity for studying gene reporter regulation and function. In this assay, firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescent system to produce light. It is designed to detect and quantitate firefly luciferase expression in mammalian cells. The kit ensures maximum sensitivity, consistency and strong bioluminescent signal.

• Reactivity:

  Mammalian

• Applications:

  Determine the effect of inhibitors or inducers in in vitro cell-based assays.,Study promoters and its interaction with transcription factors.

• Detection Method:

  Microplate luminometer

• Features & Benefits:

  An accurate, sensitive and efficient quantitation of firefly luciferase reporter enzyme activity for studying gene reporter regulation and function.,The kit ensures maximum sensitivity, consistency and strong bioluminescent signal.

• Shipping Conditions:

  Gel Pack

• Storage Conditions:

  -20°C

• Shelf Life:

  12 months