Vacuolin-1

• Description:

  Vacuolin-1 is a potent and cell-permeable lysosomal exocytosis inhibitor. Vacuolin-1 blocks the Ca2+-dependent exocytosis of lysosomes and prevents the release of lysosomal content without affecting the process of resealing. vacuolin‐1 is a potent and selective PIKfyve inhibitor and inhibits late‐stage autophagy by impairing lysosomal maturation. Vacuolin-1 can induce vacuole formation and increase the percentage of enucleated cells[1][2][4].

• UNSPSC:

  12352005

• Hazard Statement:

  H302, H315, H319, H335

• Target:

  Autophagy; PIKfyve

• Type:

  Reference compound

• Related Pathways:

  Autophagy; PI3K/Akt/mTOR

• Applications:

  COVID-19-immunoregulation

• Field of Research:

  Cancer

• Assay Protocol:

  https://www.medchemexpress.com/vacuolin-1.html

• Purity:

  98.54

• Solubility:

  DMSO : 10 mg/mL (ultrasonic; warming; heat to 60°C)

• Smiles:

  IC1=CC (/C=N/NC2=NC (N (C3=CC=CC=C3) C4=CC=CC=C4) =NC (N5CCOCC5) =N2) =CC=C1

• Molecular Formula:

  C26H24IN7O

• Molecular Weight:

  577.42

• Precautions:

  H302, H315, H319, H335

• References & Citations:

  [1]Jan Cerny, et al.The small chemical vacuolin-1 inhibits Ca (2+) -dependent lysosomal exocytosis but not cell resealing. EMBO Rep. 2004 Sep;5 (9) :883-8.|[2]Osamu Sano, et al. Vacuolin-1 inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition. FEBS Lett. 2016 Jun;590 (11) :1576-85.|[3]Yingying Lu, et al. Vacuolin-1 potently and reversibly inhibits autophagosome-lysosome fusion by activating RAB5A. Autophagy|[4]Keerthivasan G, et al. Vesicle trafficking plays a novel role in erythroblast enucleation. Blood. 2010 Oct 28;116 (17) :3331-40.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  -20°C (Powder, stored under nitrogen)

• Scientific Category:

  Reference compound1

• Clinical Information:

  No Development Reported

• CAS Number:

  351986-85-1