Anti-Rt CD8a Purified Low Endotoxin

Anti-Rt CD8a Purified Low Endotoxin

• Background: The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
• Specifications: The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal extracellular domain of rat CD8a (32-34 kDa alpha chain of the CD8 antigen).
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Rat
• Immunogen: High Mw glycoproteins from rat thymocytes
• Target Antigen: CD8a
• Isotype: Igg1 κ
• Clone: OX-8
• Applications: Flow cytometry: Recommended dilution: 1-4 µg/mL.Functional application: Blocking
• Validated Applications: WB, IHC-P, Functional assays, FC, IP, IHC-F
• Concentration: 1 mg/mL
• Format: Purified Low Endotoxin
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• References & Citations: *Pino SC, O'Sullivan-Murphy B, Lidstone EA, Yang C, Lipson KL, Jurczyk A, diIorio P, Brehm MA, Mordes JP, Greiner DL, Rossini AA, Bortell R: CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells. PLoS One. 20094(5):e5468., URL: http://www.ncbi.nlm.nih.gov/pubmed/19424493 ,*Katsumata Y, Harigai M, Sugiura T, Kawamoto M, Kawaguchi Y, Matsumoto Y, Kohyama K, Soejima M, Kamatani N, Hara M: Attenuation of experimental autoimmune myositis by blocking ICOS-ICOS ligand interaction. J Immunol. 2007 Sep 15179(6):3772-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17785814 ,*Abe Y, Urakami H, Ostanin D, Zibari G, Hayashida T, Kitagawa Y, Grisham MB: Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is required for tolerance to rat liver allografts. PLoS One. 2009 Nov 234(11):e7840., URL: https://www.ncbi.nlm.nih.gov/pubmed/19956764,*Ishida S, Usui T, Yamashiro K, Kaji Y, Amano S, Ogura Y, Hida T, Oguchi Y, Ambati J, Miller JW, Gragoudas ES, Ng YS, D'Amore PA, Shima DT, Adamis AP: VEGF164-mediated inflammation is required for pathological, but not physiological, ischemia-induced retinal neovascularization. J Exp Med. 2003 Aug 4198(3):483-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/12900522,*Mitnacht R, Bischof A, Torres-Nagel N, Hünig T: Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats. J Immunol. 1998 Jan 15160(2):700-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9551905,*Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15158(4):1833-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/9029123,*Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov22(11):2841-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1358623,*Barclay AN: The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr42(4):593-600., URL: https://www.ncbi.nlm.nih.gov/pubmed/7016746,
• Storage Conditions: Store at 2-8°C. Do not freeze.