Anti-Rt CD8a Purified

• Description:

  The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

• Certification:

  RUO

• Reactivity:

  Rat

• Immunogen:

  High Mw glycoproteins from rat thymocytes

• Target Antigen:

  CD8a

• Clone:

  OX-8

• Applications:

  FC (QC tested), IP, WB, IHC-P, IHC-F

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Pino SC, O'Sullivan-Murphy B, Lidstone EA, Yang C, Lipson KL, Jurczyk A, diIorio P, Brehm MA, Mordes JP, Greiner DL, Rossini AA, Bortell R: CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells. PLoS One. 2009;4 (5) :e5468., URL: http://www.ncbi.nlm.nih.gov/pubmed/19424493 , *Katsumata Y, Harigai M, Sugiura T, Kawamoto M, Kawaguchi Y, Matsumoto Y, Kohyama K, Soejima M, Kamatani N, Hara M: Attenuation of experimental autoimmune myositis by blocking ICOS-ICOS ligand interaction. J Immunol. 2007 Sep 15;179 (6) :3772-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17785814 , *Abe Y, Urakami H, Ostanin D, Zibari G, Hayashida T, Kitagawa Y, Grisham MB: Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is required for tolerance to rat liver allografts. PLoS One. 2009 Nov 23;4 (11) :e7840., URL: https://www.ncbi.nlm.nih.gov/pubmed/19956764, *Ishida S, Usui T, Yamashiro K, Kaji Y, Amano S, Ogura Y, Hida T, Oguchi Y, Ambati J, Miller JW, Gragoudas ES, Ng YS, D'Amore PA, Shima DT, Adamis AP: VEGF164-mediated inflammation is required for pathological, but not physiological, ischemia-induced retinal neovascularization. J Exp Med. 2003 Aug 4;198 (3) :483-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/12900522, *Mitnacht R, Bischof A, Torres-Nagel N, Hünig T: Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats. J Immunol. 1998 Jan 15;160 (2) :700-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9551905, *Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15;158 (4) :1833-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/9029123, *Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov;22 (11) :2841-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1358623, *Barclay AN: The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42 (4) :593-600., URL: https://www.ncbi.nlm.nih.gov/pubmed/7016746

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal extracellular domain of rat CD8a (32-34 kDa; alpha chain of the CD8 antigen) .

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-4 µg/ml

• Isotype:

  Mouse IgG1 kappa