Anti-Rt CD4 Purified

Anti-Rt CD4 Purified

• Background: CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1) HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1) IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
• Specifications: The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Rat
• Immunogen: MLR generated rat Th cells
• Target Antigen: CD4
• Isotype: IgG2a κ
• Clone: OX-35
• Applications: Flow cytometry: Recommended dilution: 1-4 μg/mL.
• Validated Applications: FC, IHC, ICC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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• Storage Conditions: Store at 2-8°C. Do not freeze.