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PL 7 Protein

CAT: 0247-OPPA00824-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0247-OPPA00824-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Gene Aliases
TARS, TTD7, ThrRS
Gene ID
6897
Accession Number
NP_689508.3
Reactivity
Human
Target
Threonyl-tRNA Synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 83 kDa protein is an autoantigen recognized by PL-7 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that PL-7 antibodies (similar to Jo-1 antibodies) indicate an increased risk for lung involvement, but this needs to be confirmed for a larger number of cases. TARS Human Recombinant produced in SF9 is a glycosylated, polypeptide chain.
Type
Protein
Source
Sf9 Cells
Applications
WB
Purification
Purified by proprietary chromatographic techniques.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Format
Liquid// 20mM HEPES buffer (pH 8), 200mM sodium chloride, 20% glycerol
Reconstitution
Store at 4° C if entire vial will be used within 2-4 weeks. Store, frozen at -20° C for longer periods of time. Avoid multiple freeze-thaw cycles.
Molecular Weight
83 kDa
Notes
Formerly GWB-9842E0
Protein Length
Recombinant
NCBI Gene Symbol
PL 7
Host or Source
Sf9 Insect Cells
Protein Name
Threonine--tRNA ligase, cytoplasmic
Gene Name URL
PL 7