PL 7 Protein

• Gene Aliases:

  TARS, TTD7, ThrRS

• Gene ID:

  6897

• Accession Number:

  NP_689508.3

• Reactivity:

  Human

• Target:

  Threonyl-tRNA Synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 83 kDa protein is an autoantigen recognized by PL-7 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that PL-7 antibodies (similar to Jo-1 antibodies) indicate an increased risk for lung involvement, but this needs to be confirmed for a larger number of cases. TARS Human Recombinant produced in SF9 is a glycosylated, polypeptide chain.

• Type:

  Protein

• Source:

  Sf9 Cells

• Applications:

  WB

• Purification:

  Purified by proprietary chromatographic techniques.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.5 mg/ml

• Format:

  Liquid// 20mM HEPES buffer (pH 8), 200mM sodium chloride, 20% glycerol

• Reconstitution:

  Store at 4° C if entire vial will be used within 2-4 weeks. Store, frozen at -20° C for longer periods of time. Avoid multiple freeze-thaw cycles.

• Molecular Weight:

  83 kDa

• Notes:

  Formerly GWB-9842E0

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  PL 7

• Host or Source:

  Sf9 Insect Cells

• Protein Name:

  Threonine--tRNA ligase, cytoplasmic

• Gene Name URL:

  PL 7