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PL 12 Protein

CAT: 0247-OPPA00825-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0247-OPPA00825-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Gene Aliases
AARS, CMT2N, DEE29, EIEE29
Gene ID
16
Accession Number
NP_001596.2
Reactivity
Human
Target
Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kDa protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop. AARS Human Recombinant produced in SF9 is a glycosylated, polypeptide chain.
Type
Protein
Applications
WB
Purification
Purified by proprietary chromatographic techniques.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid// 16mM HEPES buffer (pH 8), 250mM sodium chloride, 20% glycerol
Reconstitution
Store at 4° C if entire vial will be used within 2-4 weeks. Store, frozen at -20° C for longer periods of time. Avoid multiple freeze-thaw cycles.
Molecular Weight
108 kDa
Notes
Formerly GWB-95174A
Protein Length
Recombinant
NCBI Gene Symbol
PL 12
Host or Source
Sf9 Insect Cells
Protein Name
Alanine--tRNA ligase, cytoplasmic
Gene Name URL
PL 12

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