SHMT2 Recombinant Protein

• Gene Name:

  Serine hydroxymethyltransferase 2

• Gene Aliases:

  Epididymis secretory sperm binding protein Li 51e; GLY A+; GLYA; glycine auxotroph A, human complement for hamster; glycine hydroxymethyltransferase; HEL-S-51e; NEDCASB; serine aldolase; serine hydroxymethylase; serine hydroxymethyltransferase 2 (mitochondrial) ; serine hydroxymethyltransferase, mitochondrial; serine methylase; SHMT; threonine aldolase.

• Gene ID:

  6472

• Accession Number:

  NP_001159828.1

• Reactivity:

  Homo sapiens|Human

• Target:

  Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed:24075985, PubMed:29364879, PubMed:25619277) . Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed:25619277) . Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed:21876188) . Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29452640, PubMed:29364879) . Associates with mitochondrial DNA (PubMed:18063578) . In addition to its role in mitochondia, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (PubMed:24075985) .

• Conjugation:

  Unconjugated

• Type:

  Protein

• Source:

  E.coli

• Applications:

  Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  200 ug/mL (prior to lyoph)

• Format:

  Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

• Reconstitution:

  2°C to 8°C|-80°C

• Molecular Weight:

  60kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  SHMT2

• Host or Source:

  E.coli

• Protein Name:

  Serine hydroxymethyltransferase, mitochondrial

• Gene Name URL:

  SHMT2

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NM_001166356.1